货号
产品规格
售价
备注
BN42206M-50ul
50ul
¥1022.00
交叉反应:Species independent 推荐应用:WB
BN42206M-100ul
100ul
¥1360.00
交叉反应:Species independent 推荐应用:WB
英文名称 | His tag |
中文名称 | 聚组氨酸抗体标签单克隆抗体 |
别 名 | HisX6; 6His; His-Tag; 6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; Histidine. |
产品类型 | 标签抗体 |
研究领域 | 细胞生物 免疫学 |
抗体来源 | Mouse |
克隆类型 | Monoclonal |
克 隆 号 | 6E6 |
交叉反应 | Recombinant protein |
产品应用 | WB=1:5000-10000 IP=1:200-1000 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
性 状 | Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated HHHHHH peptide: |
亚 型 | IgG |
纯化方法 | affinity purified by Protein G |
储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
PubMed | PubMed |
产品介绍 | The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells. SWISS: N/A Gene ID: N/A Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 蛋白质纯化的新途径─应用His-Tag抗体检测His-Tag融合蛋白 1) 6xHis与不同的Sepharose纯化填料结合(捆绑),可不受构象的影响,在天然或变形的条件下可进行进一步纯化。 2) 可在温和的洗脱液环境中,其结合、洗涤及洗脱步骤不会对蛋白结构产生影响。 3) 6xHis标签比普通(Tag)要小得多,经纯化后的蛋白可直接进行下游操作。可用于任何表达系统(包括: 细菌,杆状病毒和哺乳动物) 4) 6xHis标签在生理溶液pH下不带电荷,其标签不会干涉重组蛋白的结构和功能, 6xHis不会影响蛋白分泌。 5) 6xHis标签免疫原性差,重组蛋白可以不必除去标签而直接作为抗原进行免疫。 |