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聚组氨酸抗体标签单克隆抗体
  • 产品货号:
    BN42206M
  • 中文名称:
    聚组氨酸抗体标签单克隆抗体
  • 英文名称:
    Mouse anti-His tag Monoclonal antibody
  • 货号

    产品规格

    售价

    备注

  • BN42206M-50ul

    50ul

    ¥1022.00

    交叉反应:Species independent 推荐应用:WB

  • BN42206M-100ul

    100ul

    ¥1360.00

    交叉反应:Species independent 推荐应用:WB

英文名称His tag
中文名称聚组氨酸抗体标签单克隆抗体
别    名HisX6; 6His; His-Tag; 6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; Histidine.  
产品类型标签抗体 
研究领域细胞生物  免疫学  
抗体来源Mouse
克隆类型Monoclonal
克 隆 号6E6
交叉反应Recombinant protein
产品应用WB=1:5000-10000 IP=1:200-1000 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
性    状Liquid
浓    度1mg/ml
免 疫 原KLH conjugated HHHHHH peptide: 
亚    型IgG
纯化方法affinity purified by Protein G
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMedPubMed
产品介绍The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.

SWISS:
N/A

Gene ID:
N/A

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

蛋白质纯化的新途径─应用His-Tag抗体检测His-Tag融合蛋白

1) 6xHis与不同的Sepharose纯化填料结合(捆绑),可不受构象的影响,在天然或变形的条件下可进行进一步纯化。
2) 可在温和的洗脱液环境中,其结合、洗涤及洗脱步骤不会对蛋白结构产生影响。
3) 6xHis标签比普通(Tag)要小得多,经纯化后的蛋白可直接进行下游操作。可用于任何表达系统(包括: 细菌,杆状病毒和哺乳动物)
4) 6xHis标签在生理溶液pH下不带电荷,其标签不会干涉重组蛋白的结构和功能, 6xHis不会影响蛋白分泌。
5) 6xHis标签免疫原性差,重组蛋白可以不必除去标签而直接作为抗原进行免疫。









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