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磷酸化核因子κB抑制蛋白α重组兔单克隆抗体
  • 产品货号：
    BN42096R
  • 中文名称：
    磷酸化核因子κB抑制蛋白α重组兔单克隆抗体
  • 英文名称：
    Rabbit anti-phospho-IKB alpha (Ser32) Monoclonal antibody

  • 货号

    产品规格

    售价

    备注

  • BN42096R-50ul

    50ul

    ¥2020.00

    交叉反应：Human(predicted:Mouse,Rat,Pig,Cow,Rabbit,Sheep) 推荐应用：WB,IHC-P,IHC-F,ICC,IF,Flow-Cyt

  • BN42096R-100ul

    100ul

    ¥3240.00

    交叉反应：Human(predicted:Mouse,Rat,Pig,Cow,Rabbit,Sheep) 推荐应用：WB,IHC-P,IHC-F,ICC,IF,Flow-Cyt

英文名称phospho-IKB alpha (Ser32)
中文名称磷酸化核因子κB抑制蛋白α重组兔单克隆抗体
别    名IKB alpha(S32); IKB alpha (phospho S32); IKB alpha (phospho Ser32); p-IKB alpha (phospho S32); I kappa B alpha; I-kappa-B-alpha; IkappaBalpha; IkB-alpha; IKBA; IKBA_HUMAN; IKBalpha; MAD 3; MAD3; Major histocompatibility complex enhancer-binding protein MAD3; NF kappa B inhibitor alpha; NF-kappa-B inhibitor alpha; NFKBI; NFKBIA; Nuclear factor of kappa light chain gene enhancer in B cells; Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor alpha.  
产品类型磷酸化抗体 
研究领域肿瘤  细胞生物  神经生物学  信号转导  激酶和磷酸酶  
抗体来源Rabbit
克隆类型Monoclonal
克 隆 号13E7
交叉反应Human,  (predicted: Mouse, Rat, Pig, Cow, Rabbit, Sheep, )
产品应用WB=1:500-1000 IHC-P=1:50-200 IHC-F=1:50-200 Flow-Cyt=2ug/Test ICC=1:50-200 IF=1:50-200 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量36kDa
细胞定位细胞核 细胞浆 
性    状Liquid
浓    度1mg/ml
免 疫 原KLH conjugated Synthesised phosphopeptide derived from human IKB alpha around the phosphorylation site of Ser32:D(p-S)GL 
亚    型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMedPubMed
产品介绍This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease. [provided by RefSeq, Aug 2011]

Function:
Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.

Subcellular Location:
Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm by a nuclear localization signal (NLS) and a CRM1-dependent nuclear export.

Post-translational modifications:
Phosphorylated; disables inhibition of NF-kappa-B DNA-binding activity. Phosphorylation at positions 32 and 36 is prerequisite to recognition by UBE2D3 leading to polyubiquitination and subsequent degradation. Sumoylated; sumoylation requires the presence of the nuclear import signal. Monoubiquitinated at Lys-21 and/or Lys-22 by UBE2D3. Ubiquitin chain elongation is then performed by CDC34 in cooperation with the SCF(FBXW11) E3 ligase complex, building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. The resulting polyubiquitination leads to protein degradation. Also ubiquitinated by SCF(BTRC) following stimulus-dependent phosphorylation at Ser-32 and Ser-36.
Deubiquitinated by porcine reproductive and respiratory syndrome virus Nsp2 protein, which thereby interferes with NFKBIA degradation and impairs subsequent NF-kappa-B activation.

DISEASE:
Defects in NFKBIA are the cause of ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant (ADEDAID) [MIM:612132]. Ectodermal dysplasia defines a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. ADEDAID is an ectodermal dysplasia associated with decreased production of pro-inflammatory cytokines and certain interferons, rendering patients susceptible to infection.

Similarity:
Belongs to the NF-kappa-B inhibitor family.
Contains 5 ANK repeats.

SWISS:
P25963

Gene ID:
4792

Database links:

Entrez Gene: 4792 Human

Omim: 164008 Human

SwissProt: P25963 Human

Unigene: 81328 Human



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.





















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