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14-3-3蛋白zeta/delta单克隆抗体
  • 产品货号：
    BN42066M
  • 中文名称：
    14-3-3蛋白zeta/delta单克隆抗体
  • 英文名称：
    Mouse anti-YWHAZ Monoclonal antibody

  • 货号

    产品规格

    售价

    备注

  • BN42066M-50ul

    50ul

    ¥2020.00

    交叉反应：Human,Rat(predicted:Mouse) 推荐应用：WB,IHC-P,IHC-F,IF

  • BN42066M-100ul

    100ul

    ¥3240.00

    交叉反应：Human,Rat(predicted:Mouse) 推荐应用：WB,IHC-P,IHC-F,IF

英文名称YWHAZ
中文名称14-3-3蛋白zeta/delta单克隆抗体
别    名Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation Protein Zeta; Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation Protein, Delta Polypeptide; Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation Protein, Zeta Polypeptide; Protein Kinase C Inhibitor Protein 1; 14-3-3 Delta; 14-3-3 Zeta; KCIP-1; Tyrosine 3/Tryptophan 5 -Monooxygenase Activation Protein, Zeta Polypeptide; 14-3-3 Protein/Cytosolic Phospholipase A2; Protein Kinase C Inhibitor Protein-1; Epididymis Secretory Protein Li 93; Epididymis Secretory Protein Li 3; Epididymis Luminal Protein 4; 14-3-3 Protein Zeta/Delta; Phospholipase A2; 14-3-3-Zeta; HEL-S-93; HEL-S-3; YWHAD;   
研究领域细胞生物  细胞凋亡  
抗体来源Mouse
克隆类型Monoclonal
克 隆 号1B6
交叉反应Human, Rat,  (predicted: Mouse, )
产品应用WB=1:500-2000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量27kDa
细胞定位细胞浆 
性    状Liquid
浓    度1mg/ml
免 疫 原Recombinant human YWHAZ protein: 
亚    型IgG2b
纯化方法affinity purified by Protein G
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMedPubMed
产品介绍This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for this gene. [provided by RefSeq, Oct 2008]

Function:
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.

Subunit:
Interacts with CDK16 and BSPRY. Interacts with WEE1 (C-terminal). Interacts with SAMSN1. Interacts with MLF1 (phosphorylated form); the interaction retains it in the cytoplasm. Interacts with Thr-phosphorylated ITGB2. Interacts with BCL2L11 (By similarity). Homodimer. Heterodimerizes with YWHAE. Homo- and hetero-dimerization is inhibited by phosphorylation on Ser-58. Interacts with FOXO4, NOXA1, SSH1 and ARHGEF2. Interacts with Pseudomonas aeruginosa exoS (unphosphorylated form). Interacts with BAX; the interaction occurs in the cytoplasm. Under stress conditions, MAPK8-mediated phosphorylation releases BAX to mitochondria. Interacts with phosphorylated RAF1; the interaction is inhibited when YWHAZ is phosphorylated on Thr-232. Interacts with TP53; the interaction enhances p53 transcriptional activity. The Ser-58 phosphorylated form inhibits this interaction and p53 transcriptional activity. Interacts with ABL1 (phosphorylated form); the interaction retains ABL1 in the cytoplasm. Interacts with PKA-phosphorylated AANAT; the interaction modulates AANAT enzymatic activity by increasing affinity for arylalkylamines and acetyl-CoA and protecting the enzyme from dephosphorylation and proteasomal degradation. It may also prevent thiol-dependent inactivation. Interacts with AKT1; the interaction phosphorylates YWHAZ and modulates dimerization. Interacts with GAB2 and TLK2. Interacts with the 'Thr-369' phosphorylated form of DAPK2 (PubMed:26047703). Interacts with PI4KB, TBC1D22A and TBC1D22B (PubMed:23572552). Interacts with ZFP36L1 (via phosphorylated form); this interaction occurs in a p38 MAPK- and AKT-signaling pathways.

Subcellular Location:
Cytoplasm.

Post-translational modifications:
The delta, brain-specific form differs from the zeta form in being phosphorylated (By similarity). Phosphorylation on Ser-184 by MAPK8; promotes dissociation of BAX and translocation of BAX to mitochondria. Phosphorylation on Thr-232; inhibits binding of RAF1. Phosphorylated on Ser-58 by PKA and protein kinase C delta type catalytic subunit in a sphingosine-dependent fashion. Phosphorylation on Ser-58 by PKA; disrupts homodimerization and heterodimerization with YHAE and TP53.

Similarity:
Belongs to the 14-3-3 family.

SWISS:
P63104

Gene ID:
7534

Database links:

Entrez Gene: 7534 Human

Entrez Gene: 22631 Mouse

Entrez Gene: 25578 Rat

Omim: 601288 Human

SwissProt: P63104 Human

SwissProt: P63101 Mouse

SwissProt: P63102 Rat

Unigene: 492407 Human

Unigene: 3360 Mouse

Unigene: 465895 Mouse

Unigene: 1292 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.


























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