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抑癌蛋白EZH2抗体
  • 产品货号：
    BN41864R
  • 中文名称：
    抑癌蛋白EZH2抗体
  • 英文名称：
    Rabbit anti-KMT6 Polyclonal antibody

  • 货号

    产品规格

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  • BN41864R-50ul

    50ul

    ¥1486.00

    交叉反应：Human,Mouse(predicted:Rat,Chicken,Dog,Pig,Cow,Horse,Rabbit) 推荐应用：IHC-P,IHC-F,ICC,IF,Flow-Cyt,ELISA

  • BN41864R-100ul

    100ul

    ¥2360.00

    交叉反应：Human,Mouse(predicted:Rat,Chicken,Dog,Pig,Cow,Horse,Rabbit) 推荐应用：IHC-P,IHC-F,ICC,IF,Flow-Cyt,ELISA

  • BN41864R-200ul

    200ul

    ¥3490.00

    交叉反应：Human,Mouse(predicted:Rat,Chicken,Dog,Pig,Cow,Horse,Rabbit) 推荐应用：IHC-P,IHC-F,ICC,IF,Flow-Cyt,ELISA

英文名称KMT6
中文名称抑癌蛋白EZH2抗体
别    名Enhancer of zeste homolog 2; Enx1h; MGC9169; Enhancer of zeste 2; ENX-1; ENX 1; ENX1; EZH1; EZH2; EZH 2; EZH2_HUMAN; Histone-lysine N-methyltransferase EZH2; KMT6A; Lysine N-methyltransferase 6; Enhancer of zeste homolog 2 (Drosophila); Enhancer of zeste, Drosophila, homolog 2; KMT 6;KMT6A; WVS2.  
研究领域肿瘤  细胞生物  免疫学  神经生物学  生长因子和激素  转录调节因子  激酶和磷酸酶  
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human, Mouse,  (predicted: Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, )
产品应用ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1ug/Test ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量82kDa
细胞定位细胞核 
性    状Liquid
浓    度1mg/ml
免 疫 原KLH conjugated synthetic peptide derived from human KMT6/EZH2:255-350/746 
亚    型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMedPubMed
产品介绍This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. This protein may play a role in the hematopoietic and central nervous systems. Multiple alternatively splcied transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Feb 2011].

Function:
Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Compared to EZH2-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes. EZH2 can also methylate non-histone proteins such as the transcription factor GATA4.

Subunit:
Binds ATRX via the SET domain (Probable). Component of the PRC2/EED-EZH2 complex, which includes EED, EZH2, SUZ12, RBBP4 and RBBP7 and possibly AEBP2. The minimum components required for methyltransferase activity of the PRC2/EED-EZH2 complex are EED, EZH2 and SUZ12. The PRC2 complex may also interact with DNMT1, DNMT3A, DNMT3B and PHF1 via the EZH2 subunit and with SIRT1 via the SUZ12 subunit. Interacts with HDAC1 and HDAC2. Interacts with PRAME.

Subcellular Location:
Nucleus.

Tissue Specificity:
Expressed in many tissues. Overexpressed in numerous tumor types including carcinomas of the breast, colon, larynx, lymphoma and testis.

Post-translational modifications:
Phosphorylated by AKT1. Phosphorylation by AKT1 reduces methyltransferase activity. Phosphorylation at Thr-345 by CDK1 and CDK2 promotes maintenance of H3K27me3 levels at EZH2-target loci, thus leading to epigenetic gene silencing.
Sumoylated.

Similarity:
Belongs to the histone-lysine methyltransferase family. EZ subfamily.
Contains 1 CXC domain.
Contains 1 SET domain.

SWISS:
Q15910

Gene ID:
2146

Database links:

Entrez Gene: 2146 Human

Entrez Gene: 14056 Mouse

Entrez Gene: 312299 Rat

Omim: 601573 Human

SwissProt: Q15910 Human

SwissProt: Q61188 Mouse

Unigene: 444082 Human

Unigene: 246688 Mouse

Unigene: 9027 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

    EZH2（enhancer of zeste homolog 2）是新识别的一种人类基因，是果蝇zeste基因增强子的人类同源基因，Polycomb group基因家族的重要成员之一。
    EZH2在多种肿瘤中高表达，具有促进细胞增殖，肿瘤细胞的扩散的恶性表型，EZH2在肿瘤中的作用成为研究热门。EZHZ在前列腺癌、乳腺癌、膀胧癌、肝细胞癌、大肠癌、胃癌中高表达;局限性前列腺癌、乳腺癌、膀胧癌高表达EZH2，则临床预后较差。因此EZH2可作为肿瘤标志物监测肿瘤的演变。随着PcG蛋白和TrxG蛋白复合物研究的深入，对细胞记忆机制的认识不断提高，而扰乱细胞的转录记忆系统可导致个体发育缺陷，使正常细胞丢失自身特性而癌变。
    EZH2通过基因沉默机制改变细胞的记忆系统并调控转录，促进前肿瘤恶性形成。EZH2在肿瘤发生发展中的作用仍远未阐明,特别是EZH2的下游基因仍不清楚,EZH2在肿瘤发生与发展的机制有待进一步研究。




























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