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载脂蛋白J抗体
  • 产品货号：
    BN41696R
  • 中文名称：
    载脂蛋白J抗体
  • 英文名称：
    Rabbit anti-APOJ Polyclonal antibody

  • 货号

    产品规格

    售价

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  • BN41696R-50ul

    50ul

    ¥1486.00

    交叉反应：Rat,Human(predicted:Mouse) 推荐应用：IHC-P,IHC-F,IF,ELISA

  • BN41696R-100ul

    100ul

    ¥2360.00

    交叉反应：Rat,Human(predicted:Mouse) 推荐应用：IHC-P,IHC-F,IF,ELISA

  • BN41696R-200ul

    200ul

    ¥3490.00

    交叉反应：Rat,Human(predicted:Mouse) 推荐应用：IHC-P,IHC-F,IF,ELISA

英文名称APOJ
中文名称载脂蛋白J抗体
别    名AAG 4; AAG4 ;Aging associated protein 4; Clusterin-alpha/beta; APOJ; Apo J; Apolipoprotein J; Cli; CLU; Clusterin alpha chain; Clusterin; Clusterin beta chain; Complement associated protein SP 40 40; Complement associated protein SP 40; Complement associated protein SP40; Complement cytolysis inhibitor; Complement lysis inhibitor; KUB 1; KUB1; MGC24903; NA1/NA2; SGP 2; SGP2; SP 40; SP40; Sulfated glycoprotein 2; Testosterone repressed prostate message 2; TRPM 2; TRPM2; Clusterin- Alpha/Beta; Apolipoprotein J.  




研究领域肿瘤  心血管  细胞生物  免疫学  脂蛋白  
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human, Mouse, Rat, 
产品应用WB=1:100-500 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量50kDa
细胞定位分泌型蛋白 
性    状Liquid
浓    度1mg/ml
免 疫 原KLH conjugated synthetic peptide derived from human APO J:331-449/449 
亚    型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMedPubMed
产品介绍Apolipoprotein J is the major secreted product of Sertoli cells and is thought to play a critical role in spermatogenesis. The protein was shown to be a normal constituent of human blood and is also thought to be a control mechanism of the complement cascade. It prevents the binding of a C5b-C7 complex to the membrane of the target cell and in this way inhibits complement-mediated cytolysis.
Apolipoprotein J is overexpressed in human prostate and breast cancers and in squamous cell carcinomas. Suppression of Apolipoprotein J renders these cells sensitive to chemotherapeutic drug-mediated apoptosis. It has been proposed that elevated levels of Apolipoprotein J may promote oncogenic transformation and tumor progression by interfering with BAX proapoptotic activities.

Function:
Isoform 1 functions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress-induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. Secreted isoform 1 protects cells against apoptosis and against cytolysis by complement. Intracellular isoforms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. Nuclear isoforms promote apoptosis. Mitochondrial isoforms suppress BAX-dependent release of cytochrome c into the cytoplasm and inhibit apoptosis. Plays a role in the regulation of cell proliferation.

Subcellular Location:
Nucleus. Cytoplasm. Mitochondrion membrane. Cytoplasm, cytosol. Microsome. Endoplasmic reticulum. Cytoplasmic vesicle, secretory vesicle, chromaffin granule. Isoforms lacking the N-terminal signal sequence have been shown to be cytoplasmic and/or nuclear. Secreted isoforms can retrotranslocate from the secretory compartments to the cytosol upon cellular stress. Detected in perinuclear foci that may be aggresomes containing misfolded, ubiquitinated proteins. Detected at the mitochondrion membrane upon induction of apoptosis and Secreted. Can retrotranslocate from the secretory compartments to the cytosol upon cellular stress.

Tissue Specificity:
Detected in blood plasma, cerebrospinal fluid, milk, seminal plasma and colon mucosa. Detected in the germinal center of colon lymphoid nodules and in colon parasympathetic ganglia of the Auerbach plexus (at protein level). Ubiquitous. Detected in brain, testis, ovary, liver and pancreas, and at lower levels in kidney, heart, spleen and lung.

Post-translational modifications:
Isoform 1 is proteolytically cleaved on its way through the secretory system, probably within the Golgi lumen. Polyubiquitinated, leading to proteasomal degradation.
Heavily N-glycosylated. About 30% of the protein mass is comprised of complex N-linked carbohydrate.

Similarity:
Belongs to the clusterin family.

SWISS:
P10909

Gene ID:
1191

Database links:

Entrez Gene: 1191 Human

Entrez Gene: 12759 Mouse

Entrez Gene: 24854 Rat

Omim: 185430 Human

SwissProt: P10909 Human

SwissProt: Q06890 Mouse

SwissProt: P05371 Rat

Unigene: 436657 Human

Unigene: 200608 Mouse

Unigene: 1780 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.


























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