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谷氨酸脱羧酶67抗体
  • 产品货号：
    BN41684R
  • 中文名称：
    谷氨酸脱羧酶67抗体
  • 英文名称：
    Rabbit anti-GAD67 Polyclonal antibody

  • 货号

    产品规格

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  • BN41684R-50ul

    50ul

    ¥1486.00

    交叉反应：Human,Mouse(predicted:Rat) 推荐应用：WB,IHC-P,IHC-F,IF,Flow-Cyt,ELISA

  • BN41684R-100ul

    100ul

    ¥2360.00

    交叉反应：Human,Mouse(predicted:Rat) 推荐应用：WB,IHC-P,IHC-F,IF,Flow-Cyt,ELISA

  • BN41684R-200ul

    200ul

    ¥3490.00

    交叉反应：Human,Mouse(predicted:Rat) 推荐应用：WB,IHC-P,IHC-F,IF,Flow-Cyt,ELISA

英文名称GAD67
中文名称谷氨酸脱羧酶67抗体
别    名glutamate decarboxylase 67; decarboxylase 1; 67 kDa glutamic acid decarboxylase; Glutamate decarboxylase 67 kDa isoform; GAD1; GAD; GAD-67; GAD 67.  
研究领域肿瘤  细胞生物  免疫学  神经生物学  新陈代谢  
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human, Mouse,  (predicted: Rat, )
产品应用WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量67kDa
细胞定位细胞浆 细胞膜 
性    状Liquid
浓    度1mg/ml
免 疫 原KLH conjugated synthetic peptide derived from human GAD67:455-594/594 
亚    型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMedPubMed
产品介绍Glutamic Acid Decarboxylase (GAD) catalyzes the conversion of L glutamate to g-aminobutyric acid (GABA), the principal inhibitory neurotransmitter in the brain, and a putative paracrine signal molecule in pancreatic islets. GAD has a restricted tissue distribution. It is highly expressed in the cytoplasm of GABAergic neurons in the central nervous system (CNS) and pancreatic beta cells. It is also present in other non-neuronal tissues such as testis, oviduct and ovary. GAD is also transiently expressed in non-GABAergic cells of the embryonic and adult nervous system, suggesting its involvement in development and plasticity.
GAD exists as two isoforms, GAD65 and GAD67 (molecular masses of 65 and 67 kD, respectively) that are encoded by two different genes. GAD65 is an ampiphilic, membraneanchored protein, (585 amino acid residues) and is encoded on human chromosome 10. GAD67 is a cytoplasmic protein (594 amino acid residues) and is encoded on chromosome 2. There is 64% amino acid identity between the two isoforms, with the highest diversity located at the N terminus, which in GAD65 is required for targeting the enzyme to GABA-containing secretory vesicles. The two isoforms appear to have distinct intraneuronal distribution in the brain. GAD65 has been identified as an autoantigen in insulindependent diabetes mellitus (IDDM) and stiff-man syndrome (SMS), IDDM is an autoimmune disease that results from T cell mediated destruction of pancreatic insulin-secreting beta cells. Islet-reactive T cells and antibodies primarily to GAD65 (also named beta cell autoantigen) can be detected in peripheral blood of 80% of recent-onset IDD patients and in pre-diabetic high-risk subjects before onset of clinical symptoms. This suggests that GAD may be an important marker in the early stages of the disease. Also, autoantibodies to GAD65 and GAD67 are detected in animal models of IDDM, including the non-obese diabetes (NOD) mouse. In the NOD mouse, T cell reactivity is initially restricted to the C terminal regions of GAD65, but later spreads to other parts of GAD65. Stiff-man syndrome (SMS), a rare disorder of the CNS, is characterized by progressive rigidity of the body musculature with painful spasms, due to impairment of the GABAergic neurotransmission.

Function:
Catalyzes the production of GABA.

Subcellular Location:
Mitochondrion; Plasma Membrane;

Tissue Specificity:
Isoform 3 is expressed in pancreatic islets, testis, adrenal cortex, and perhaps other endocrine tissues, but not in brain.

DISEASE:
Defects in GAD1 are the cause of cerebral palsy spastic quadriplegic type 1 (CPSQ1) [MIM:603513]. A non-progressive disorder of movement and/or posture resulting from defects in the developing central nervous system. Affected individuals manifest symmetrical, non-progressive spasticity and no adverse perinatal history or obvious underlying alternative diagnosis. Developmental delay, mental retardation and sometimes epilepsy can be part of the clinical picture.

Similarity:
Belongs to the group II decarboxylase family.

SWISS:
Q99259

Gene ID:
2571

Database links:

Entrez Gene: 2571 Human

Entrez Gene: 14415 Mouse

Entrez Gene: 24379 Rat

Omim: 605363 Human

SwissProt: Q99259 Human

SwissProt: P48318 Mouse

SwissProt: P18088 Rat

Unigene: 420036 Human

Unigene: 272120 Mouse

Unigene: 91245 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

GAD67谷氨酸脱羧酶-67 是用于I II型糖尿病研究的很重要的蛋白。GAD67的大多数表位位于蛋白中部或C末端1／3蛋白，与GAD65有高度的同源性。


























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