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半胱氨酸蛋白酶8抗体
  • 产品货号：
    BN41474R
  • 中文名称：
    半胱氨酸蛋白酶8抗体
  • 英文名称：
    Rabbit anti-Caspase 8 Polyclonal antibody

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  • BN41474R-50ul

    50ul

    ¥1486.00

    交叉反应：Human,Rat,Sheep(predicted:Mouse,Chicken,Dog,Pig,Cow) 推荐应用：IHC-P,IHC-F,ICC,IF,Flow-Cyt,ELISA

  • BN41474R-100ul

    100ul

    ¥2360.00

    交叉反应：Human,Rat,Sheep(predicted:Mouse,Chicken,Dog,Pig,Cow) 推荐应用：IHC-P,IHC-F,ICC,IF,Flow-Cyt,ELISA

  • BN41474R-200ul

    200ul

    ¥3490.00

    交叉反应：Human,Rat,Sheep(predicted:Mouse,Chicken,Dog,Pig,Cow) 推荐应用：IHC-P,IHC-F,ICC,IF,Flow-Cyt,ELISA

英文名称Caspase 8
中文名称半胱氨酸蛋白酶8抗体
别    名Caspase-8 p10; Caspase-8 subunit p10; ALPS2B; Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 12 protein; Apoptosis related cysteine peptidase; Apoptotic cysteine protease; Apoptotic protease Mch 5; Apoptotic protease Mch-5; Apoptotic protease Mch5; CAP 4; CAP4; CASP 8; CASP-8; Caspase-8; Caspase8; CASP8; CASP8_HUMAN; Caspase8; Caspase 8 apoptosis related cysteine peptidase; Caspase8; CED 3; FADD homologous ICE/CED 3 like protease; FADD Homologous ICE/CED3 Like Protease; FADD Like ICE antibody FADD-homologous ICE/CED-3-like protease; FADD-like ICE; FLICE; FLJ17672; ICE like apoptotic protease 5; ICE-like apoptotic protease 5; MACH alpha 1/2/3 protein; MACH antibody MACH beta 1/2/3/4 protein antibody MCH 5; MCH5.  






研究领域细胞生物  神经生物学  细胞凋亡  
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human, Rat, Sheep,  (predicted: Mouse, Chicken, Dog, Pig, Cow, )
产品应用ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test ICC=1:100 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量12/55kDa
细胞定位细胞浆 
性    状Liquid
浓    度1mg/ml
免 疫 原KLH conjugated synthetic peptide derived from rat Caspase-8 subunit p10:411-482/482 
亚    型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMedPubMed
产品介绍Caspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. Caspases have the ability to cleave after aspartic acid residues. There are two classes of caspases involved in apoptosis; initiators (activation by receptor cluster) and effectors (activation by mitochondrial permeability transition). Proapoptotic signals autocatalytically activate initiator caspases, such as Caspase 8 and Caspase 9. Activated initiator caspases then process effector caspases, such as Caspase 3 and Caspase 7, which in turn cause cell collapse.

Function:
Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-|-AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoform 5, isoform 6, isoform 7 and isoform 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex.

Subunit:
Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 18 kDa (p18) and a 10 kDa (p10) subunit. Interacts with FADD, CFLAR and PEA15. Isoform 9 interacts at the endoplasmic reticulum with a complex containing BCAP31, BAP29, BCL2 and/or BCL2L1. Interacts with TNFAIP8L2.

Subcellular Location:
Cytoplasm.

Tissue Specificity:
Isoform 1, isoform 5 and isoform 7 are expressed in a wide variety of tissues. Highest expression in peripheral blood leukocytes, spleen, thymus and liver. Barely detectable in brain, testis and skeletal muscle.

DISEASE:
Defects in CASP8 are the cause of caspase-8 deficiency (CASP8D) [MIM:607271]. CASP8D is a disorder resembling autoimmune lymphoproliferative syndrome (ALPS). It is characterized by lymphadenopathy, splenomegaly, and defective CD95-induced apoptosis of peripheral blood lymphocytes (PBLs). It leads to defects in activation of T-lymphocytes, B-lymphocytes, and natural killer cells leading to immunodeficiency characterized by recurrent sinopulmonary and herpes simplex virus infections and poor responses to immunization.

Similarity:
Belongs to the peptidase C14A family.
Contains 2 DED (death effector) domains.

SWISS:
O89110

Gene ID:
841

Database links:

Entrez Gene: 841 Human

Entrez Gene: 12370 Mouse

Entrez Gene: 54474 Rat

Entrez Gene: 64044 Rat

Omim: 601763 Human

SwissProt: Q14790 Human

SwissProt: O89110 Mouse

SwissProt: Q9JHX4 Rat

Unigene: 599762 Human

Unigene: 655983 Human

Unigene: 336851 Mouse



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
























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