货号
产品规格
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BN41432M-100ul
100ul
¥1360.00
交叉反应:Species independent 推荐应用:WB,ELISA
英文名称 | TAP-Tag |
中文名称 | TAP-Tag(标签)单克隆抗体 |
别 名 | TAP Tag; TAPTag; Tandem affinity purification |
产品类型 | 标签抗体 |
抗体来源 | Mouse |
克隆类型 | Monoclonal |
克 隆 号 | Mix-mA™ |
交叉反应 | Recombinant protein |
产品应用 | WB=1:1000-5000 ELISA=1:5000-10000 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
性 状 | Liquid |
浓 度 | 1mg/ml |
免 疫 原 | Recombinant TAP-Tag: |
亚 型 | IgG |
纯化方法 | affinity purified by Protein G |
储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
PubMed | PubMed |
产品介绍 | Tandem affinity purification (TAP) is a purification technique for studying protein–protein interactions. It involves creating a fusion protein with a designed piece, the TAP tag, on the end. The original TAP method involves the fusion of the TAP tag to the C-terminus of the protein under study. The TAP tag consists of calmodulin binding peptide (CBP) from the N-terminal, followed by tobacco etch virus protease (TEV protease) cleavage site and Protein A, which binds tightly to IgG. The relative order of the modules of the tag is important because Protein A needs to be at the extreme end of the fusion protein so that the entire complex can be retrieved using an IgG matrix. SWISS: N/A Gene ID: N/A Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |