首页>>蛋白研究>>抗体>>丝氨酸/苏氨酸蛋白激酶SRPK2抗体
丝氨酸/苏氨酸蛋白激酶SRPK2抗体
  • 产品货号：
    BN41288R
  • 中文名称：
    丝氨酸/苏氨酸蛋白激酶SRPK2抗体
  • 英文名称：
    Rabbit anti-SRPK2 Polyclonal antibody

  • 货号

    产品规格

    售价

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  • BN41288R-100ul

    100ul

    ¥2360.00

    交叉反应：Human,Mouse(predicted:Rat,Chicken,Dog,Pig,Cow,Horse,Rabbit,Sheep) 推荐应用：IHC-P,IHC-F,IF,ELISA

  • BN41288R-200ul

    200ul

    ¥3490.00

    交叉反应：Human,Mouse(predicted:Rat,Chicken,Dog,Pig,Cow,Horse,Rabbit,Sheep) 推荐应用：IHC-P,IHC-F,IF,ELISA

英文名称SRPK2
中文名称丝氨酸/苏氨酸蛋白激酶SRPK2抗体
别    名Human serine kinase SRPK2 mRNA, complete cds; Serine kinase SRPK2; Serine/arginine rich protein specific kinase 2; Serine/threonine protein kinase SRPK2; SFRS protein kinase 2; SFRSK2; SR protein specific kinase 2; SRPK2_HUMAN.  
研究领域细胞生物  信号转导  细胞周期蛋白  激酶和磷酸酶  表观遗传学  
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human, Mouse,  (predicted: Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep, )
产品应用ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量77kDa
细胞定位细胞核 细胞浆 
性    状Liquid
浓    度1mg/ml
免 疫 原KLH conjugated synthetic peptide derived from human SRPK2:266-350/688 
亚    型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMedPubMed
产品介绍SRPK2 belongs to the protein kinase superfamily. It phosphorylates RS domain-containing proteins, such as SFRS1 and SFRS2 on serine residues. It has a role in spliceosome assembly and in mediating the trafficking of splicing factors and appears to mediate HBV core protein phosphorylation which is a prerequisite for pregenomic RNA encapsidation into viral capsids. SRPK2 highly expressed in brain, moderately expressed in heart and skeletal muscle and at low levels in lung, liver, and kidney.

Function:
Serine/arginine-rich protein-specific kinase which specifically phosphorylates its substrates at serine residues located in regions rich in arginine/serine dipeptides, known as RS domains and is involved in the phosphorylation of SR splicing factors and the regulation of splicing. Promotes neuronal apoptosis by up-regulating cyclin-D1 (CCND1) expression. This is done by the phosphorylation of SRSF2, leading to the suppression of p53/TP53 phosphorylation thereby relieving the repressive effect of p53/TP53 on cyclin-D1 (CCND1) expression. Phosphorylates ACIN1, and redistributes it from the nuclear speckles to the nucleoplasm, resulting in cyclin A1 but not cyclin A2 up-regulation. Plays an essential role in splicesomal B complex formation via the phosphorylation of DDX23/PRP28. Can mediate hepatitis B virus (HBV) core protein phosphorylation. Plays a negative role in the regulation of HBV replication through a mechanism not involving the phosphorylation of the core protein but by reducing the packaging efficiency of the pregenomic RNA (pgRNA) without affecting the formation of the viral core particles.

Subunit:
Interacts with PKB/AKT1 in a phosphorylation-dependent manner. The phosphorylated form (by PKB/AKT1) interacts with YWHAB and YWHAE. Interaction with YWHAB suppresses its cleavage by caspases and inhibits the release of its N-terminal pro-apoptotic fragment. Interacts with SFN. Associates with U4/U6-U5 tri-small nuclear ribonucleoproteins (U4/U6-U5 tri-snRNPs).

Subcellular Location:
Cytoplasm. Nucleus. Note=Shuttles between the nucleus and the cytoplasm. KAT5/TIP60 inhibits its nuclear translocation. Phosphorylation at Thr-492 by PKB/AKT1 promotes nuclear translocation.

Tissue Specificity:
Highly expressed in brain, moderately expressed in heart and skeletal muscle and at low levels in lung, liver, and kidney.

Post-translational modifications:
Proteolytically cleaved at Asp-139 and Asp-403 by caspase-3 during apoptotic cell death. Cleavage at Asp-139 which is the major site of cleavage, produces a small N-terminal fragment that translocates into nucleus and promotes VP16-induced apoptosis.

Similarity:
Phosphorylation at Thr-492 by PKB/AKT1 enhances its stimulatory activity in triggering cyclin-D1 (CCND1) expression and promoting apoptosis in neurons, which can be blocked by YWHAB. It also enhances its protein kinase activity toward ACIN1 and SRSF2, promotes its nuclear translocation and prevents its proteolytic cleavage.
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family.
Contains 1 protein kinase domain.

SWISS:
P78362

Gene ID:
6733

Database links:

Entrez Gene: 6733 Human

Entrez Gene: 20817 Mouse

Entrez Gene: 296753 Rat

Omim: 602980 Human

SwissProt: P78362 Human

SwissProt: O54781 Mouse

Unigene: 285197 Human

Unigene: 288728 Mouse



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

























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