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细胞分裂周期相关蛋白5抗体
  • 产品货号：
    BN41266R
  • 中文名称：
    细胞分裂周期相关蛋白5抗体
  • 英文名称：
    Rabbit anti-CDCA5 Polyclonal antibody

  • 货号

    产品规格

    售价

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  • BN41266R-100ul

    100ul

    ¥2360.00

    交叉反应：Rat,Mouse,Human(predicted:Rabbit,Horse,Cow,Pig,Dog) 推荐应用：IHC-P,IHC-F,IF,Flow-Cyt,ELISA

  • BN41266R-200ul

    200ul

    ¥3490.00

    交叉反应：Rat,Mouse,Human(predicted:Rabbit,Horse,Cow,Pig,Dog) 推荐应用：IHC-P,IHC-F,IF,Flow-Cyt,ELISA

英文名称CDCA5
中文名称细胞分裂周期相关蛋白5抗体
别    名Cell division cycle associated protein 5; MGC16386; p35; Sororin; CDCA5_HUMAN.  
研究领域细胞生物  染色质和核信号  细胞周期蛋白  细胞分化  表观遗传学  
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human, Mouse,  (predicted: Rat, Dog, Pig, Cow, Horse, Rabbit, )
产品应用ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=3ug/Test IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量28kDa
细胞定位细胞核 
性    状Liquid
浓    度1mg/ml
免 疫 原KLH conjugated synthetic peptide derived from human CDCA5:101-200/252 
亚    型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMedPubMed
产品介绍Sororin, also designated cell division cycle-associated protein 5 (CDCA5) or p35, functions as a regulator of sister chromatid cohesion during mitosis. It interacts with the APC/C complex and is found in a complex consisting of cohesion components SCC-112, MC1L1, SMC3L1, RAD21 and APRIN. The deduced human and mouse Sororin proteins consist of 252 and 264 amino acid residues, respectively, and both contain a KEN box for APC-dependent ubiquitination. Reserach demonstrates a punctated nuclear distribution of Sororin during interphase and a diffuse distribution throughout the cell during mitosis. There is no apparent concentration of Sororin on chromatin in mitotic cells and Sororin levels decrease in synchronized HeLa cells during interphase. The Sororin gene maps to chromosome 11q12.1.

Function:
CDCA5 is a regulator of sister chromatid cohesion in mitosis. It may act by regulating the ability of the cohesin complex to mediate sister chromatid cohesion, perhaps by altering the nature of the interaction of cohesin with the chromosomes. CDCA5 interacts with the APC/C complex and is found in a complex with the cohesin components SMC1L1, SMC3L1, RAD21, PDS5A/APRIN and PDS5B/SCC-112.

Subunit:
Interacts with the APC/C complex (By similarity). Interacts with the chromatin-bound cohesin complex; the interaction is indirect, occurs after DNA replication and requires acetylation of the cohesin component SMC3. Interacts (via the FGF motif) with PDS5A and PDS5B; the interaction is direct and prevents the interaction of PDS5A with WAPAL.

Subcellular Location:
Nuclear.

Post-translational modifications:
Phosphorylated. Phosphorylation, as cells enter mitosis, disrupts the interaction with PDS5A and relieves the inhibition of WAPAL by CDCA5.
Ubiquitinated by the APC/C complex in G1, leading to its degradation (Probable).

Similarity:
Belongs to the sororin family.

SWISS:
Q96FF9

Gene ID:
113130

Database links:
UniProtKB/Swiss-Prot: Q96FF9.1

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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