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甲基化CpG结合蛋白抗体
  • 产品货号：
    BN41176R
  • 中文名称：
    甲基化CpG结合蛋白抗体
  • 英文名称：
    Rabbit anti-MBD1 Polyclonal antibody

  • 货号

    产品规格

    售价

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  • BN41176R-100ul

    100ul

    ¥2360.00

    交叉反应：Human,Rat(predicted:Mouse) 推荐应用：WB,IHC-P,IHC-F,IF,ELISA

  • BN41176R-200ul

    200ul

    ¥3490.00

    交叉反应：Human,Rat(predicted:Mouse) 推荐应用：WB,IHC-P,IHC-F,IF,ELISA

英文名称MBD1
中文名称甲基化CpG结合蛋白抗体
别    名CXXC 3; CXXC3; MBD 1; MBD1; MECP1 COMPLEX; Methyl CpG binding domain protein 1; Methyl CpG binding domain protein 1 isoform PCM1; Methyl CpG binding protein MBD1; Methyl CpG binding protein splice variant 1; Methyl CpG binding protein splice variant 2; Methyl CpG binding protein splice variant 3; Methyl CpG binding protein splice variant 4; PCM 1; PCM1; Protein containing methyl CpG binding domain 1; Regulator of fibroblast growth factor 2 (FGF 2) transcription; RFT; The regulator of fibroblast growth factor 2(FGF 2) transcription; MBD1_HUMAN.  
研究领域细胞生物  免疫学  染色质和核信号  细胞周期蛋白  细胞分化  
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human, Rat,  (predicted: Mouse, )
产品应用WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量60kDa
细胞定位细胞核 
性    状Liquid
浓    度1mg/ml
免 疫 原KLH conjugated synthetic peptide derived from human MBD1:101-200/605 
亚    型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMedPubMed
产品介绍DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD). The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA. MBD2 and MBD4 (also known as MED1) were later found to colocalize with foci of heavily methylated satellite DNA and believed to mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. MBD4 have homology to bacterial base excision repair DNA N-glycosylases/lyases. In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract.

Subcellular Location:
Nucleus speckle. Chromosome.

Tissue Specificity:
Widely expressed.

Similarity:
Contains 3 CXXC-type zinc fingers.
Contains 1 MBD (methyl-CpG-binding) domain.

SWISS:
Q9UIS9

Gene ID:
4152

Database links:

Entrez Gene: 4152 Human

Omim: 156535 Human

SwissProt: Q9UIS9 Human

Unigene: 405610 Human



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications















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