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检查点蛋白HUS1抗体
  • 产品货号：
    BN40509R
  • 中文名称：
    检查点蛋白HUS1抗体
  • 英文名称：
    Rabbit anti-HUS1 Polyclonal antibody

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    产品规格

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  • BN40509R-100ul

    100ul

    ¥2360.00

    交叉反应：Rat(predicted:Human,Mouse,Pig,Cow,Horse) 推荐应用：IHC-P,IHC-F,ICC,IF,ELISA

  • BN40509R-200ul

    200ul

    ¥3490.00

    交叉反应：Rat(predicted:Human,Mouse,Pig,Cow,Horse) 推荐应用：IHC-P,IHC-F,ICC,IF,ELISA

英文名称HUS1
中文名称检查点蛋白HUS1抗体
别    名Checkpoint protein HUS1; hHUS1; HUS1 (S. pombe) checkpoint homolog; Hus1; HUS1 checkpoint homolog (S. pombe); HUS1 Checkpoint Protein; HUS1 protein; HUS1+ - like protein; HUS1_HUMAN; Hydroxyurea-sensitive 1, S. pombe, homolog of.  
研究领域细胞生物  转录调节因子  表观遗传学  
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Rat,  (predicted: Human, Mouse, Pig, Cow, Horse, )
产品应用ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量32kDa
细胞定位细胞核 细胞浆 
性    状Liquid
浓    度1mg/ml
免 疫 原KLH conjugated synthetic peptide derived from human HUS1:51-150/280 
亚    型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMedPubMed
产品介绍The protein encoded by this gene is a component of an evolutionarily conserved, genotoxin-activated checkpoint complex that is involved in the cell cycle arrest in response to DNA damage. This protein forms a heterotrimeric complex with checkpoint proteins RAD9 and RAD1. In response to DNA damage, the trimeric complex interacts with another protein complex consisting of checkpoint protein RAD17 and four small subunits of the replication factor C (RFC), which loads the combined complex onto the chromatin. The DNA damage induced chromatin binding has been shown to depend on the activation of the checkpoint kinase ATM, and is thought to be an early checkpoint signaling event. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2011]

Function:
Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates.

Subcellular Location:
Nucleus. Cytoplasm. In discrete nuclear foci upon DNA damage. According to PubMed:14500360, localized also in the cytoplasm. DNA damage induces its nuclear translocation. Shuttles between the nucleus and the cytoplasm.

Tissue Specificity:
Ubiquitous.

Similarity:
Belongs to the HUS1 family.

SWISS:
O60921

Gene ID:
3364

Database links:

Entrez Gene: 3364 Human

Omim: 603760 Human

SwissProt: O60921 Human

Unigene: 152983 Human



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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